Protein Domain : Pre-mRNA-splicing factor Cwc2, RNA recognition motif IPR034181

Type	Domain
Description	This entry represents the RNA recognition motif (RRM) of yeast protein Cwc2, also termed Cwf2, one of the components of the Prp19-associated complex [nineteen complex (NTC)]. that can bind to RNA. NTC is composed of the scaffold protein Prp19 and a number of associated splicing factors, and plays a crucial role in intron removal during premature mRNA splicing in eukaryotes. Cwc2 functions as an RNA-binding protein that can bind both small nuclear RNAs (snRNAs) and pre-mRNA in vitro. It interacts directly with the U6 snRNA to link the NTC to the spliceosome during pre-mRNA splicing []. In the N-terminal half, Cwc2 contains a CCCH-type zinc finger (Znf domain), a RNA recognition motif (RRM), and an intervening loop, also termed RNA-binding loop or RB loop, between the Znf and the RRM, all of which are necessary and sufficient for RNA binding. The Znf is also responsible for mediating protein-protein interaction. The C-terminal flexible region of Cwc2 interacts with the WD40 domain of Prp19 [ ].
Short Name	Cwc2_RRM