Protein Domain : Gamma-adaptin ear (GAE) domain IPR008153

Type	Domain
Description	The adaptor proteins AP-1 and GGA (Golgi-localized, gamma ear-containing, ADP- ribosylation factor (ARF)-binding proteins) regulate membrane traffic betweenthe trans-Golgi network (TGN) and endosome/lysosomes through ARF-regulated membrane association, recognition of sorting signals, and recruitment ofclathrin and accessory proteins. The gamma-adaptin ear (GAE) domain is a C- terminal appendage or ear of about 120 residues, which is found in gamma-adaptins, the heavy subunits of the AP-1 complex, and in GGAs. The GAE domain, which is found in associated with other domains such as VHS,coiled-coils and GAT, is involved in the recruitment of accessory proteins, such as gamma-synergin, Rababptin-5, Eps15 and cyclin G-associated kinase,which modulate the functions of GAE domain containing proteins in the membrane trafficking events [, , , ].The resolution of the 3D-structure of the human gamma-adaptin GAE domain shows that it forms an immunoglobulin-like β-sandwich fold composed of eight β-strands with two short α-helices. The topology ofthe entire GAE domain is similar to those of the N-terminal subdomains in the alpha- and beta-adaptin ear domains of the AP-2 complex. However, the GAEdomain has very low sequence identity and homology to the N-terminal immunoglobulin-like subdomains of the alpha and beta ear domains. The bindingsite for the accessory proteins has been located to a shallow hydrophobic trough surrounded by charged (mainly basic) residues [, ].This entry represents the entire GAE domain.
Short Name	GAE_dom