GWAS : mixed.gwas.Han_Zhao_2015

Synopsis  440 soybean landraces and elite cultivars were screened for resistance to SCN HG Type 0 and HG Type 1.2.3.5.7; GWAS relative to Wm82.gnm2
Genotyping Platform  Han_Zhao_2015
Genotypes  440 diverse soybean landraces and elite cultivars mostly collected in China.
Description  The Genomic DNA of the 440 accessions were partially sequenced using specific-locus amplified fragment sequencing (SLAF-seq) approach by Illumina Genome Analyzer II. The 347 million paired-end reads with 45 bp read length were mapped to the reference soybean gen- ome (Wm82.a2.v1) and a mean of 57,418 high quality tags were identified from paired-end reads for each accession. A data set of 36,976 SNPs at Minor Allele Fre- quency (MAF)≥4 % was generated from 57,418 high quality tags. The rate of missing data was controlled at less than 10 % for each accession. The set of SNPs covered 20 chromosomes of soybean. The resulting SNP density of one SNP per 27 Kbp was available in the present study.
Genotyping Method  Specific Locus Amplified Fragment Sequencing (SLAF-seq)

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